The NEBNext ARTIC kits were developed in response to the critical need for reliable and accurate methods for sequencing SARS-CoV-2.

• Updated primers for effective Omicron coverage
• Effective with a wide range of viral genome inputs (10 to 10000 copies)
• Express protocol option, with fewer cleanups

These kits, for long and short read sequencing, were based on the original work of the ARTIC Network. The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach. NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation. The balanced primers and reagents for RT-PCR deliver improved uniformity of amplicon yields from gRNA across a wide copy number range. The NEBNext ARTIC SARS-CoV-2 Companion Library Prep Kit (Oxford Nanopore Technologies) is compatible with a broad range of total RNA input amounts and is used alongside kits from Oxford Nanopore Technologies to generate targeted, high quality libraries for next-generation sequencing on the Oxford Nanopore platform.