The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template.
- Prepare RNA-seq libraries from extremely low input: single cells/nuclei or 2 pg of total RNA
- Perform 5' RACE from as low as 10 ng total RNA
- Low background for RNA-seq or 5' RACE
- Enzyme mix contains Murine RNase Inhibitor, no additives necessary
These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
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- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
: VWR International
: VWR International
: VWR International
: VWR International