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Specifications
- Source:Human
- Species:Human
- Size:5 mg
- Storage conditions:Store unopened product at 2-8 °C. Store opened material no longer than 1-2 days at 2-8 °C unless sodium azide is added. Do not freeze.
- Protein/peptide name:IgM
- Purity:>99%, liquid
- Concentration:Total Protein: 5.3 mg/mL; Volume: 19.4 mL
- Tested applications:Immunoassays
- Cat. no.:IC0855916
Specifications
About this item
Product is human IgM and buffer salts. From human myeloma, highly purified, immunoabsorbed to remove trace amounts of contaminant proteins.
- Standard in ELISA (100 μg/ml)
- Dot blot assay
- Purified human IgM is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
- From human myeloma, highly purified, immunoabsorbed to remove trace amounts of contaminant proteins.
The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is human IgM; no trace of IgG, IgA or non-immunoglobulin serum proteins is detected. The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 5 mg of human IgM is recovered.
Human IgM is purified from human myeloma serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 μm filter and vialed. No preservative has been added.