Dulbecco's phosphate buffered saline is used for tissue disaggregation and monolayer dispersal.The presence of calcium and magnesium ions hinder the trypsin activity.

• Off-white to creamish white
• pH: 7.20 to 7.80
• Osmolality without sodium bicarbonate: 275.00 to 315.00 mOsm
• Endotoxin content: NMT 1 EU/ml

The solution is used to maintain the medium within physiological pH range and intracellular and extra cellular osmotic balance. Modified with a carbohydrate, such as glucose serves as an energy source for cell metabolism.