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Specifications
- Description:Kit contains: Hypotonic Buffer, Dithiothreitol, Inhibitor Cocktail, PBS, Phosphatase Inhibitors, Extraction Buffer, Reagent
- Cat. no.:75816-726
- Supplier No.:10009277-1
Specifications
About this item
For the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates
- Isolate nuclear and cytoplasmic fractions from cell lysates and tissue homogenates
- Resulting isolates can be used in Transcription Factor Assay Kits
- Isolates can also be used for EMSA or western blotting
- Each kit provides reagents for 50 extractions from approximately 10 million cells
Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman’s Nuclear Extraction is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman’s Transcription Factor Assay kits. The proteins isolated using this kit can also be used in electrophoretic mobility shift assays (EMSA) and western blotting applications.