About this item
EMS Shield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
- Fortified with DAPI a counter-stain for DNA
- Does not contain phenylenediamine
- Refractive Index in solution: 1.364 ± 0.002
The unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phycocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4 °C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI. May encounter problems with frozen brain or other frozen tissues with lots of fat. Applications in immunofluorescence and confocal microscopy. Reagent: Ready to use mounting medium.
Storage: 2-8°C, Protect from light, DO NOT FREEZE.
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Supplier: VWR International
Supplier: VWR International
Supplier: BDH
Supplier: VWR International
Supplier: Corning
Supplier: VWR International