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Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific
Catalog # CAPI34070
Supplier:  Thermo Scientific
CAS Number:  
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Pierce 1-Step™ NBT/BCIP plus Suppressor Substrate Solution, Thermo Scientific
Catalog # CAPI34070
Supplier:  Thermo Scientific
Supplier Number:  34070
CAS Number:  
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Specifications

  • Description:
    1-Step™ NBT/BCIP Plus Suppressor Solution
  • Size:
    100 mL
  • Cat. no.:
    CAPI34070
  • Application:
    Immunoblotting
  • Supplier No.:
    34070
  • No. of Uses:
    10 uses at 10 ml each

Specifications

About this item

The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

  • Sensitive with low background and minimial assay-to-assay variability
  • Ready-to-use single component

The combination of NBT and BCIP yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, an enzyme conjugate for antibody probes. Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase. Used for immunoblotting, immunohistochemistry, and in situ hybridization applications.