To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
- Description:1-Step™ NBT/BCIP Plus Suppressor Solution
- Size:100 mL
- Cat. no.:CAPI34070
- Application:Immunoblotting
- Supplier No.:34070
- No. of Uses:10 uses at 10 ml each
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
- Sensitive with low background and minimial assay-to-assay variability
- Ready-to-use single component
The combination of NBT and BCIP yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, an enzyme conjugate for antibody probes. Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase. Used for immunoblotting, immunohistochemistry, and in situ hybridization applications.