The Mem-PER™ Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure

• Extraction and isolation - produces minimal cross-contamination (typically less than 10%) of hydrophilic protein into the hydrophobic (membrane) protein fraction
• Cells or tissues - effect for extraction from cultured mammalian and yeast cells and mammalian tissues
• Downstream compatibility - analyze membrane protein extracts by SDS-PAGE, western blotting, and protein assays
• Fast and simple - isolation membrane proteins in approximately one hour

This kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure. Hydrophobic proteins having three or fewer membrane-spanning domains are effectively solubilized and isolated from hydrophilic (cytoplasmic) proteins using the three-reagent system.