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Y-PER™ reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.
- Suitable for use in a diverse range of situations
- Extracts more than twice as much protein as glass bead methods
- Eliminates the physical problems associated with traditional glass bead lysis
- Effective for many different organisms, including Gram-positive and Gram-negative bacteria
This reagent have been optimized to encompass a broad array of applications ranging from fusion-tagged protein purification to microplate-compatible enzyme assays and genomic and plasmid DNA extraction from yeast
Additional components, such as protease inhibitors, salt, or chelating agents may be added to the reagent depending upon applications.
Also, it has been used to isolate yeast killer virus double - stranded RNA from killer strains of Saccharomyces cerevisiae.
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