Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- Antigen retriever for some antibodies in IHC
- Please refer to primary antibody protocol for IHC
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC.
Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
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- Storage conditions:2 – 8 °C
- Storage temperature:2-8°C. DO NOT FREEZE
: VWR International
: VWR International
: VWR International
: VWR International