Competitive ELISA for quantitation of PGD2 in biological samples
- Treatment of samples with MOX hydrochloride converts unstable PGD2 to stable PGD2-MOX
- Highly sensitive
- Measure PGD2-MOX levels down to 3.1 pg/ml
- Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
- Midpoint: 50% B/B0: 10-35 pg/ml
This PGD2-MOX ELISA is based on the conversion of PGD2 to a stable MOX derivative. Treatment of the sample with MOX HCl converts PGD2 into PGD2-MOX, preventing its further chemical degradation. The antiserum used in the assay was developed using conjugates of this derivative and is very specific for PGD2-MOX.
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- Assay Type:Competitive
- Detection range:2.0 – 250 pg/ml
- Sensitivity:80% B/B0: 3 pg/mL
: VWR International
: VWR International
: VWR International
: VWR International