Protein Carbonyl Colorimetric Assay Kit, Cayman Chemical Company
75816-674
:
- Brand name:CAYM
- Cat. no.:75816-674
- Contents:Kit contains: Hydrochloric Acid, DNPH, Solution, Guanidine Hydrochloride, Ethanol, Ethyl Acetate, and Plate
- Supplier No.:10005020-96
Convenient colorimetric assay for measurement of oxidized proteins.
- Measure protein carbonyl content in plasma, serum, urine, tissue homogenates, and cell lysates
- Assay 24 samples in duplicate
- Plate-based colorimetric measurement (360 – 385 nm)
The carbonyls of protein samples are derivatized using 2,4-dinitrophenylhydrazine. Formation of a Schiff base produces the corresponding hydrazone which can be analyzed spectrophotometrically at 360 – 385 nm. This assay can be used to measure oxidized protein in plasma, serum, cell lysates, and tissue homogenates.