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- Description:SensoLyte® 520 Cathepsin S Assay Kit *Fluorimetric*
- Size:1 Kit
- Storage temperature:-20°C
- Cat. no.:103010-420
- No. of Wells:96
- No. of Assays/Kit:100 assays
- Supplier No.:AS-72099
Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases
Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities.
The cathepsin assay kits offers a series of fluorimetric long wavelength, sensitive SensoLyte® 520 assay kits for enzyme activity determination of Cathepsins G, D, E, B, K, L and S. These kits employ a FRET (Fluorescence Resonance Energy Transfer) peptide substrate that emits in the green range of 520 nm, with excitation at 490 nm. The advantage of using longer wavelength emitting substrates means that autofluorescence from cellular components and test compounds is minimal; thus making the kits more sensitive. Furthermore, the use of HiLyte Fluor™ 488 (except for Cathepsin S, which uses 5-FAM) as the FRET donor (fluorophore) results in a more stable fluorescent signal, since HiLyte Fluor™ 488 is resistant to acidic pH. A listing of the SensoLyte® Cathepsin Kits providing conventional FRET (Mca/Dnp) or fluorogenic substrates for cathepsins are also available.
Kits detect specific Cathepsin activities; provides ample reagents to perform 100 assays in 96-well format; minimal hands-on time required; detailed protocol and references provided.
SensoLyte® 520 Cathepsin S Activity Assay Kit uses a 5-FAM/QXL®520 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the 5-FAM/QXL® 520 based FRET peptide shows less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The kit can be used to detect the activity of Cathepsin S enzyme in biological samples and purified enzyme preparations.