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Specifications
- Description:SensoLyte® FDP Alkaline Phosphatase Assay Kit *Fluorimetric*
- Size:1 Kit
- Storage temperature:4 °C
- Cat. no.:103010-122
- No. of Wells:96
- No. of Assays/Kit:500 assays
- Supplier No.:AS-71109
Specifications
About this item
Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses
- Features: Kits include all essential components (chromogenic/fluorogenic/luminometry substrates) and sample reagents needed to perform 500 assays using 96-well plate format.
- Optimized to detect specific alkaline phosphatase activity; compatible for HTS; detailed protocol and references provided.
Phosphatases are important classes of enzymes involved in cellular signal transduction, cell metabolism and other biological activities. Changes in alkaline phosphatase level and activity are involved in a variety of physiological and pathological events, such as bone development, bone-related diseases, gestation related diseases, inflammatory bowel disease, post-parathyroidectomy stage and drug toxicity. Phosphatases have attracted considerable interest as: Enzyme markers, Drug screening targets, Conjugating agents and Reporter for gene expression studies.
The SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes).