Pierce™ Blocker™ BSA Blocking Buffer, Thermo Scientific

À propos de cet article

Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Easy to use
Convenient and saves storage space
Purified protein provides fewer chances of cross reaction with assay components

This 10% (w/v) solution of high quality BSA that is useful for saturating excess protein binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) in 1X Tris-buffered saline for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than non fat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.

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