Pierce™ Melon™ Gel IgG Spin Purification Kits, Thermo Scientific
CAPI45206
:
- Description:Melon™ Gel IgG Spin Purification Kit
- Size:1 kit (3 ml gel)
- Cat. no.:CAPI45206
- Supplier No.:45206
The Pierce™ Melon™ Gel IgG Spin Purification Kit is a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.
- Higher recovery and purity
- Effective for subclasses of IgG that bind poorly to Protein A or G
- Regenerable, support can be regenerated and used for up to 6 purifications
- Compatible, amine free buffer does not require removal or neutralization
The IgG is isolated in the one-step protocol up to six times faster than Protein A or G purification methods. The protocol does not require any desalting or extreme pH conditions, enabling antibodies to retain their biological properties. Melon™ gel consistently offers better than a 90% recovery rate with better than 80% for all antibodies, including some subclasses that are generally unattainable using Protein A or G purification methods.
: 3 ml Gel up to 25 x 0.1 mL serum samples (total approx. 25 mg)
: Kit includes Melon™ Gel support (3 mL); Melon™ Gel Purification Buffer (dry mix to make 100X), (100 mL); Spin Columns (0.8 mL, 27 columns); Microcentrifuge Tubes (2 mL, 30 tubes).