Rosetta™ 2 Singles™ Competent Cells, EMD Millipore
About this item
A common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
- Hosts carry a chromosomal copy of the T7 RNA polymerase gene
- Strains are suitable for production of protein from target genes
- Target genes are cloned in T7 expression vectors, using IPTG as an inducer
- Guaranteed efficiency: >2×106 cfu/µg
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lac UV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3).
4 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
A common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
- Hosts carry a chromosomal copy of the T7 RNA polymerase gene
- Strains are suitable for production of protein from target genes
- Target genes are cloned in T7 expression vectors, using IPTG as an inducer
- Guaranteed efficiency: >2×106 cfu/µg
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lac UV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3).
Product Family Options
Product Information
- DésignationTailleIncludesPackagingAvailabilityPrice
- Rosetta™ 2(DE3) Singles™ Competent Cells11 Reactions2×2 ml SOC Medium
10 µl Test PlasmidPlastic ampouleLog in for availabilityEach$581.15Supplier Number: 71400-3Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. - Rosetta™ 2(DE3) Singles™ Competent Cells22 Reactions4×2 ml SOC Medium
10 µl Test PlasmidGlass bottleLog in for availabilityEach$1,048.54Supplier Number: 71400-4Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. - Rosetta™ 2(DE3)pLysS Singles™ Competent Cells11 Reactions2×2 ml SOC Medium
10 µl Test PlasmidPlastic ampouleLog in for availabilityEach$581.15Supplier Number: 71401-3Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing. - Rosetta™ 2(DE3)pLysS Singles™ Competent Cells22 Reactions4×2 ml SOC Medium
10 µl Test PlasmidGlass bottleLog in for availabilityEach$1,048.54Supplier Number: 71401-4Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
#: CA21905-026
Supplier: Kimberly-Clark