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- Cat. no.:75817-392
- Includes:Kit contains: Buffer, Reagents, and 96-Well Plate
- Supplier No.:709001-96
For measurement of the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysate samples.
- Measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates
- Assay 41 samples in duplicate
- Measure antioxidant capacity in Trolox equivalents down to 44 μM
- Plate-based colorimetric measurement (750 or 405 nm)
The antioxidant system of living organisms includes enzymes such as superoxide dismutase, catalase, and glutathione peroxidase; macromolecules such as albumin, ceruloplasmin, and ferritin; and an array of small molecules, including ascorbic acid, α-tocopherol, β-carotene, reduced glutathione, uric acid, and bilirubin
The Cayman Chemical Antioxidant Assay Kit measures the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2'-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS® · + by metmyoglobin. The capacity of the antioxidants in the sample to prevent ABTS oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as molar Trolox equivalents. Each kit contains assay buffer (10X), chromogen, metmyoglobin, trolox, hydrogen peroxide, a 96 well plate, plate cover, and complete instructions.
The sum of endogenous and food-derived antioxidants represents the total antioxidant activity of the extracellular fluid. Cooperation of all the different antioxidants provides greater protection against attack by reactive oxygen or nitrogen radicals, than any single compound alone. Thus, the overall antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present in plasma and body fluids.