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- Description:HRP Detection Reagent
- Size:25 mL
- Method:OD570 nm, FL530/585 nm
- Sample:Biological, compatible with all species
- Processing time:30 minutes
- Cat. no.:75878-124
- Supplier No.:QFHRP-25ML
Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for HRP detection.
- For both absorbance or fluorescence detection of horse-radish peroxidase activity
- Highly sensitive, convenient and fast
QuantiFluo™ Horseradish Peroxidase Reagent is a stable 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent formulated for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent product (λex/em = 530/590nm) when reacted with H2O2 by a peroxidase. The fluorescent product can also be visualized under a light source (530 ± 20 nm).