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Specifications
- Description:Pyruvate Kinase Assay Kit
- Size:100 assays
- Method:OD570 nm, FL530/585 nm
- Sample:Plasma, serum, cell and tissue etc
- Processing time:40 minutes
- Cat. no.:75878-164
- Supplier No.:EPRK-100
Specifications
About this item
For quantitative determination of pyruvate kinase activity.
- Linear detection range 0.1 - 50 U/L for colorimetric assays and 0.01 - 2 U/L for fluorimetric assays
Pyruvate Kinase (PK) is an enzyme involved in glycolysis
BioAssay Systems’ Pyruvate Kinase Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity. In this assay PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/590nm is directly proportional to the pyruvate generated by the Pyruvate Kinase in the sample.
It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, a genetic disease, is caused by a lack of pyruvate kinase and slows down the process of glycolysis. Pyruvate kinase is also involved in gluconeogenesis, a biochemical pathway in which the liver generates glucose from pyruvate and other substrates.