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Specifications
- Description:Alcohol Dehydrogenase Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Biological (e.g. plasma, serum, urine, tissue and culture media).
- Processing time:30 minutes
- Cat. no.:75877-950
- Supplier No.:DADH-100
Specifications
About this item
For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.
- Linear detection range 0.4 to 80 U/L for 30 min reaction
- Detection limit of 0.1 U/L for 120 min reaction
- This homogeneous "mix-incubate-measure" type assay can be readily automated for processing thousands of samples per day
Alcohol dehydrogenase (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones
BioAssay Systems’ non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.