À propos de cet article
Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
- Ideal for conjugating cysteine-containing peptide antigens to make immunogen or screening target
- Conjugation reaction complete in 2 hours; results in stable, covalent thioether bonds
- Single polypeptide protein with molecular mass of 45000 Daltons
- Contains 14 aspartic acid and 33 glutamic acid residues whose carboxyl groups account for the net negative charge (pI 4.63) of OVA
Imject™ Ovalbumin is not as immunogenic as other proteins, such as keyhole limpet hemocyanin (KLH) but more soluble in DMSO (dimethyl sulfoxide) for applications that require this solvent. It is useful as an irrelevant protein carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten.
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