Human Recombinant UBE2A (from E. coli)
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Product Details & Documents
Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC.
- Fusion-Tag: N-GST, 6 His tag
This recombinant protein can be used for biological assays. For research use only.
Spécifications
Spécifications
- Taille:0.05 mg
- Protein/peptide name:UBE2A
- Protein synonyms:Ubiquitin Carrier Protein A|RAD6 Homolog A|Ubiquitin-Conjugating Enzyme E2 A|RAD6A|hHR6A|HR6A|UBE2A|Ubiquitin-Protein Ligase A
- Purity:> 95% as determined by reducing SDS-PAGE
- Sequence:Met1-Cys152
- Source:E. coli
- Molecular weight:49 kD
- Cat. No.:75789-042
- Protein/peptide type:Recombinant
- Endotoxin content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
- Formulation:Supplied as a 0.2 um filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tag sequence:N-GST, 6 His tag
- Tested applications:Bioassay
- Species:Human
Spécifications
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