The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

• High Expression with Up to 750 µg/ml of Expressed Protein
• Low Variability between Experiments
• Express High Levels of Protein with 6 Hours to Overnight Incubation
• Obtain Functionally Active Proteins, Including those Containing Disulfide Bonds
• Transcription and Translation is Performed in One Reaction Step
• Amenable to Incorporation of Heavy or Unnatural Amino Acids

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/ml. The complete mini-scale kit includes all the components required for transcription and translation of a recombinant gene, including an optimised expression vector.