The Pierce™ Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

• Procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
• Provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
• Provides higher viability than do-it-yourself methods and other commercial kits
• Cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

The Pierce™ Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.