The Thermo Scientific Pierce™ classic IP kit uses high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays with ease.

• Spin columns eliminate resin loss and provide efficient separation of solutions
• Immunoprecipitate (IP) in less than one hour
• Recover antigen without harsh detergents or reducing agents
• Purify target protein from crude lysate in four simple steps

The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies

Like traditional IP methods, the Pierce Classic IP Kit procedure involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin (Protein A/G Agarose). After washing to remove nonbound (presumably undesired) components of the sample, the antigen and antibody are recovered from the beaded resin with elution buffer supplied in the kit. The entire procedure is performed in a microcentrifuge spin column, allowing solutions to be fully separated from the agarose resin upon brief centrifugation.

The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.