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Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific
  CAPI20158
 :  Thermo Scientific
 :  
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Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific
  CAPI20158
 :  Thermo Scientific
 :  20158
 :  
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  • Description:
    LightShift™ Chemiluminescent RNA EMSA Kit
  • Cat. no.:
    CAPI20158
  • For Membrane Area:
    1000 cm² (10 mini gel blots)
  • No. of Reactions:
    100
  • Supplier No.:
    20158

 

 

The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

  • Chemiluminescent detection comparable to radioactive detection
  • Develop X-ray films after 1 to 5 minute exposures thus being a time saving process
  • Compatible with RNA probes biotinylated by different methods
  • Complete kit includes optimized reagents for binding reactions and RNA probe detection
  • Eliminate waste concerns from radioactive RNA probes

Chemiluminescent RNA EMSA Kit is an in vitro technique for detection of protein-RNA interactions through changes in gel electrophoresis migration patterns. This kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods.

 : Kit includes 125 nM Biotinylated IRE RNA control (35 µL), 10 µM unlabeled IRE RNA control (25 µL), 2 mg/mL Cytosolic liver extract (50 µL), 10 mg/mL tRNA (100 µL), 10X REMSA binding buffer (1 mL), 50% Glycerol (500 µL), 2M KCl (500 µL), 1M MgCl2 (500 µL), DTT lyophilized, nuclease-free water (1,5 mL), 5X REMSA loading buffer (1 mL), Stabilized streptavidin-HRP (1,5 mL), Luminol/enhancer solution (80 mL), Stable peroxide solution (80 mL), Nucleic acid detection blocking buffer (500 mL), 4X wash buffer (500 mL), and Substrate equilibration buffer (500 mL).
 : For Research Use Only. Not for use in diagnostic procedures.