Pierce™ LightShift™ Chemiluminescent RNA EMSA Kit, Thermo Scientific

Catalog # CAPI20158

Supplier: Thermo Scientific

CAS Number:

Catalog # CAPI20158

Supplier: Thermo Scientific

Supplier Number: 20158

CAS Number:

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Specifications

Description:
LightShift™ Chemiluminescent RNA EMSA Kit
Cat. no.:
CAPI20158
For Membrane Area:
1000 cm²(10 mini gel blots)
No. of Reactions:
100
Supplier No.:
20158

Specifications

About this item

The Thermo Scientific LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

Chemiluminescent detection comparable to radioactive detection
Develop X-ray films after 1 to 5 minute exposures thus being a time saving process
Compatible with RNA probes biotinylated by different methods
Complete kit includes optimized reagents for binding reactions and RNA probe detection
Eliminate waste concerns from radioactive RNA probes

Chemiluminescent RNA EMSA Kit is an in vitro technique for detection of protein-RNA interactions through changes in gel electrophoresis migration patterns. This kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional ³²P-isotopic methods.

Delivery: Kit includes 125 nM Biotinylated IRE RNA control (35 µL), 10 µM unlabeled IRE RNA control (25 µL), 2 mg/mL Cytosolic liver extract (50 µL), 10 mg/mL tRNA (100 µL), 10X REMSA binding buffer (1 mL), 50% Glycerol (500 µL), 2M KCl (500 µL), 1M MgCl₂ (500 µL), DTT lyophilized, nuclease-free water (1,5 mL), 5X REMSA loading buffer (1 mL), Stabilized streptavidin-HRP (1,5 mL), Luminol/enhancer solution (80 mL), Stable peroxide solution (80 mL), Nucleic acid detection blocking buffer (500 mL), 4X wash buffer (500 mL), and Substrate equilibration buffer (500 mL).

Caution: For Research Use Only. Not for use in diagnostic procedures.