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- Description:SuperSignal™ West HisProbe Kit, sufficient for 62 mini blots or 5000 cm2 membrane
- Size:685 mL
- Cat. no.:CAPI15168
- Supplier No.:15168
The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by Western blotting
- One-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
- HisProbe-HRP can recognize his-tags independent of adjacent tags
- Smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies
- Economical, considerably less costly than anti-6xHis-tag antibodies
The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).
HisProbe-HRP Conjugate is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.
HisProbe-HRP Conjugate, 2 mg
SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
SuperSignal West Pico Stable Peroxide Solution, 250 mL
Blocker BSA in TBS (10X), 125 mL
TBS Packs (each makes 500 mL), 10 packs
Surfact-Amps 20 Detergent Solution, 6 x 10 mL
Store HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4?C. Store remaining components at 4?C.