Human Recombinant LIF (from E. coli)
About this item
LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.6 kDa protein containing 180 amino acids residues, including three disulfide bonds. Manufactured using all Animal-Free reagents.
- Carrier free
- Animal-free
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.
6 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.6 kDa protein containing 180 amino acids residues, including three disulfide bonds. Manufactured using all Animal-Free reagents.
- Carrier free
- Animal-free
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 10778-588
- 10778-590
- 10778-592
- 10778-594
- 10778-596
- 10778-598
- Protein/peptide name:
- LIF
- LIF
- LIF
- LIF
- LIF
- LIF
- Protein/peptide type:
- Recombinant
- Recombinant
- Recombinant
- Recombinant
- Recombinant
- Recombinant
- Species:
- Human
- Human
- Human
- Human
- Human
- Human
- Source:
- E. coli
- E. coli
- E. coli
- E. coli
- E. coli
- E. coli
- Animal-free:
- Y
- Y
- Y
- Y
- Y
- Y
- Endotoxin content:
- <0.1 ng/μg of protein (<1 EU/μg).
- <0.1 ng/μg of protein (<1 EU/μg).
- <0.1 ng/μg of protein (<1 EU/μg).
- <0.1 ng/μg of protein (<1 EU/μg).
- <0.1 ng/μg of protein (<1 EU/μg).
- <0.1 ng/μg of protein (<1 EU/μg).
- Biological activity:
- Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Purity:
- Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Greater than 98% by SDS-PAGE gel and HPLC analyses.
- Formulation:
- Lyophilized
- Lyophilized
- Lyophilized
- Lyophilized
- Lyophilized
- Lyophilized
- Storage conditions:
- −20 °C
- −20 °C
- −20 °C
- −20 °C
- −20 °C
- −20 °C
- Shipping temperature:
- RT
- RT
- RT
- RT
- RT
- RT
Specifications
Product Family Options
Product Information
- TailleOriginAvailabilityPrice
- Supplier Number: AF-300-05-5UGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10778-588Protein/peptide nameLIFProtein/peptide typeRecombinantSpeciesHumanSourceE. coliAnimal-freeYEndotoxin content<0.1 ng/μg of protein (<1 EU/μg).Biological activityDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.PurityGreater than 98% by SDS-PAGE gel and HPLC analyses.FormulationLyophilizedStorage conditions−20 °CShipping temperatureRT - Supplier Number: AF-300-05-25UGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10778-590Protein/peptide nameLIFProtein/peptide typeRecombinantSpeciesHumanSourceE. coliAnimal-freeYEndotoxin content<0.1 ng/μg of protein (<1 EU/μg).Biological activityDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.PurityGreater than 98% by SDS-PAGE gel and HPLC analyses.FormulationLyophilizedStorage conditions−20 °CShipping temperatureRT - Supplier Number: AF-300-05-100UGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10778-592Protein/peptide nameLIFProtein/peptide typeRecombinantSpeciesHumanSourceE. coliAnimal-freeYEndotoxin content<0.1 ng/μg of protein (<1 EU/μg).Biological activityDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.PurityGreater than 98% by SDS-PAGE gel and HPLC analyses.FormulationLyophilizedStorage conditions−20 °CShipping temperatureRT - Supplier Number: AF-300-05-250UGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10778-594Protein/peptide nameLIFProtein/peptide typeRecombinantSpeciesHumanSourceE. coliAnimal-freeYEndotoxin content<0.1 ng/μg of protein (<1 EU/μg).Biological activityDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.PurityGreater than 98% by SDS-PAGE gel and HPLC analyses.FormulationLyophilizedStorage conditions−20 °CShipping temperatureRT - Supplier Number: AF-300-05-500UGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10778-596Protein/peptide nameLIFProtein/peptide typeRecombinantSpeciesHumanSourceE. coliAnimal-freeYEndotoxin content<0.1 ng/μg of protein (<1 EU/μg).Biological activityDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.PurityGreater than 98% by SDS-PAGE gel and HPLC analyses.FormulationLyophilizedStorage conditions−20 °CShipping temperatureRT - Supplier Number: AF-300-05-1MGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10778-598Protein/peptide nameLIFProtein/peptide typeRecombinantSpeciesHumanSourceE. coliAnimal-freeYEndotoxin content<0.1 ng/μg of protein (<1 EU/μg).Biological activityDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.PurityGreater than 98% by SDS-PAGE gel and HPLC analyses.FormulationLyophilizedStorage conditions−20 °CShipping temperatureRT
#: CA21905-026
Supplier: Kimberly-Clark