IBI DNA/RNA/Protein Extraction Kit, IBI Scientific
À propos de cet article
The DNA/RNA/Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids
DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20 – 30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.
The quality of the DNA/RNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1×106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.
Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.
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Product Details & Documents
The DNA/RNA/Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids
DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20 – 30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.
The quality of the DNA/RNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1×106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.
Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.
Specifications for Product Family
Spécifications
Spécifications
- Catalog No:
- 10304-254
- 10304-256
- 10304-258
- Refine target molecule:
- DNA|Total RNA|Total protein
- DNA|Total RNA|Total protein
- DNA|Total RNA|Total protein
- Sample type:
- Blood|Cultured cells
- Blood|Cultured cells
- Blood|Cultured cells
- Prep size:
- Midi
- Midi
- Midi
- Sample size:
- Cultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)
- Cultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)
- Cultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)
- Yield:
- up to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction) - up to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction) - up to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction)
Spécifications
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Informations sur le produit
- DésignationTailleAvailabilityPrice
- Supplier Number: IB47700Spécifications:
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Specifications
Cat. No.10304-254Refine target moleculeDNA|Total RNA|Total proteinSample typeBlood|Cultured cellsPrep sizeMidiSample sizeCultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)Yieldup to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction) - Supplier Number: IB47701Spécifications:
More
Specifications
Cat. No.10304-256Refine target moleculeDNA|Total RNA|Total proteinSample typeBlood|Cultured cellsPrep sizeMidiSample sizeCultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)Yieldup to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction) - Supplier Number: IB47702Spécifications:
More
Specifications
Cat. No.10304-258Refine target moleculeDNA|Total RNA|Total proteinSample typeBlood|Cultured cellsPrep sizeMidiSample sizeCultured animal cells (up to 5x10^6), up to 25mg of tissue, up to 500 µl of whole human blood, up to 200 µl of biological liquids (serum, plasma)Yieldup to 9 µg of Genomic DNA from 1.5x10^6 HeLa cell
20 µg of Total RNA from 1.5x10^6 HeLa cells
120 µg of protein from 1.5x10^6 HeLa cells
Operation tiime: 25 minutes (DNA/RNA Extraction); 50 minutes (Protein Extraction)
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