AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step

• High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
• Stringent, ultrapure antibody hotstart enables precise target amplification and ambient room temperature setup

Activated AccuStart II Taq DNA Polymerase possesses 5' ̶ ˃3’ DNA polymerase activity and a double-strand specific 5’ ̶ ˃3’ exonuclease. The polymerase does not have 3’-exonuclease activity and is free of any contaminating endo or exonuclease activities. One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. AccuStart™ II Taq DNA polymerase contains extremely low levels of residual host, E. coli genomic DNA.

AccuStart II Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.

Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimized buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.