Heart Lysate, ProSci
À propos de cet article
Heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
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- L'article nécessite un contrôle de la température pour le stockage et la livraison avec des frais supplémentaires. Il n'est pas éligible au retour pour des raisons de sécurité et de qualité. Tenez compte des exigences avant d'acheter.
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Product Details & Documents
Heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Specifications for Product Family
Spécifications
Spécifications
- Catalog No:
- 10062-130
- 10062-248
- 10062-630
- Concentration:
- 2 mg/mL
- 2 mg/mL
- 2 mg/mL
- Tested applications:
- WB
- WB
- WB
- Storage buffer:
- Heart lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Heart lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Heart lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Storage conditions:
- Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
Spécifications
Product Family Options
Informations sur le produit
- DésignationFormat de celluleTailleSourceTissueAvailabilityPrice
- Supplier Number: 1301L'article nécessite un contrôle de la température pour le stockage et la livraison avec des frais supplémentaires. Il n'est pas éligible au retour pour des raisons de sécurité et de qualité. Tenez compte des exigences avant d'acheter.Spécifications:
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Specifications
Cat. No.10062-130Concentration2 mg/mLTested applicationsWBStorage bufferHeart lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.Storage conditionsStore at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. - Supplier Number: 1461L'article nécessite un contrôle de la température pour le stockage et la livraison avec des frais supplémentaires. Il n'est pas éligible au retour pour des raisons de sécurité et de qualité. Tenez compte des exigences avant d'acheter.Spécifications:
More
Specifications
Cat. No.10062-248Concentration2 mg/mLTested applicationsWBStorage bufferHeart lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.Storage conditionsStore at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. - Supplier Number: 1401L'article nécessite un contrôle de la température pour le stockage et la livraison avec des frais supplémentaires. Il n'est pas éligible au retour pour des raisons de sécurité et de qualité. Tenez compte des exigences avant d'acheter.Spécifications:
More
Specifications
Cat. No.10062-630Concentration2 mg/mLTested applicationsWBStorage bufferHeart lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.Storage conditionsStore at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
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Catalog #: CA21905-026
Supplier: Kimberly-Clark