JB-4™ Embedding Kit, Electron Microscopy Sciences

A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalyzed monomer acts as a dehydration and infiltration agent. Therefore, complete dehydration through 100% ethanol is not necessary although recommended for large or dense tissues. Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4.JB-4 Kit Consists of 800 mL Solution A, 30 mL Solution B, and 12 g Catalyst.

Thin sections (.5-2.0 micron) with excellent morphological structure preservation
Water-clear blocks; casts in 90 minutes max. at room temp
Good lipid and enzyme retention when processing at low temperatures (4°C)
Removal of JB-4 resin prior to staining is not necessary
Clearing agents such as xylene and chloroform are not needed
Higher clarity and contrast than with paraffin sections
Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue
It produces harder blocks which is ideal for dense samples, such as bone

Thin sections (.5-2.0 micron) with excellent morphological structure preservation
Water-clear blocks; casts in 90 minutes max. at room temp
Good lipid and enzyme retention when processing at low temperatures (4°C)
Removal of JB-4 resin prior to staining is not necessary
Clearing agents such as xylene and chloroform are not needed
Higher clarity and contrast than with paraffin sections
Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue
It produces harder blocks which is ideal for dense samples, such as bone

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