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- Description:K.lactis Protein Expression Kit
- Cat. no.:CA101294-866
The K
The gene is cloned into the integrative pKLAC-series of vectors, and may be expressed intracellularly or secreted. The K. lactis system offers several advantages over other yeast and bacterial expression systems. Abundant overexpression of protein is achieved through high culture densities as well as the ability to integrate multiple copies of the vector. The pKLAC-series of vectors use a strong LAC4 promoter, which has been modified to lack expression in E. coli, making this system useful for expressing toxic genes. The high transformation efficiency of the supplied K. lactis cells makes the system suitable for methods that require large numbers of transformants, such as expression cloning using cDNA libraries. For the selection of transformants, no expensive antibiotics are required. In addition, no methanol is required in growth media. Finally, the K. lactis system can express post-translationally modified proteins, making it a useful alternative to bacterial expression systems.
lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis.