TAT Broth

For the cultivation of microorganisms from highly viscous or gelatinous materials.

• Microorganism cultivation
• Diagnosis of human disease
• Contains preservatives for self-sterilization against vegetative bacteria, yeast, and mold

Hardy Diagnostics TAT (Tryptone-Azolectin-Tween®) Broth is recommended for use in cultivating microorganisms from highly viscous or gelatinous materials, such as salves and ointments.

This product is not intended to be used for the diagnosis of human disease.

TAT (Tryptone-Azolectin-Tween) Broth, also known as Casein-peptone Lecithin Polysorbate Broth, is recommended for sterility testing of viscous materials, such as salves, creams and ointments, and is especially suited to the sterility testing of cosmetics. Cosmetics and topical pharmaceutical products are easily subjected to contamination during the manufacturing process and through continued use by consumers. Consequently, these products contain preservatives for self-sterilization against vegetative bacteria, yeast, and mold.

Hardy Diagnostics TAT Broth formulations are enriched to isolate and cultivate microorganisms from viscous materials. These media contain pancreatic digest of casein, which provides nitrogen, vitamins, amino acids, and carbon. Lecithin (azolectin) and Tween® 20 neutralize common preservatives in cosmetics or pharmaceutical products, thereby permitting the growth of contaminating flora. The relatively high casein content provides optimal conditions for spore germination and regeneration of damaged cells. Lecithin and polysorbate 20 inactivate many antimicrobial compounds.

These media are especially adapted to the microbiological examination of non-sterile products from pharmaceutical, cosmetic and raw material or end-products, for the purpose of performing microbial enumeration studies.