EE Broth Mossel

For use in the selective enrichment, isolation, and enumeration of Enterobacteriaceae, particularly from foods. Conforms with harmonized USP/EP/JP requirements.

• Contains glucose to facilitate the growth of most Enterobacteriaceae
• EE Broth Mossel, USP should be used as an enrichment broth
• Contains peptones as a source of nitrogen, vitamins and amino acids

EE Broth Mossel, USP conforms to the formulation as specified in the U.S

The detection of Enterobacteriaceae is of great concern in the microbial examination of non-sterile products and in monitoring the sanitary quality of foods. Enterobacteriaceae are easily injured during processing procedures, exposure to low temperatures, sub-marginal heat, drying, radiation, and preservatives or sanitizers. Therefore, the ability to recover these microorganisms depends upon proper resuscitation of sub-lethally damaged cells.

EE Broth Mossel, USP contains peptones as a source of nitrogen, vitamins and amino acids. Dextrose provides a carbon source. Phosphates act as buffering agents, while brilliant green and oxbile (oxgall) are added as selective agents.

Pharmacopoeia (USP), which is based on the formula proposed by Mossel, Visser and Cornelissen. The formula contains glucose to facilitate the growth of most Enterobacteriaceae and aids in the detection of Salmonella and other lactose-negative organisms. EE Broth Mossel, USP should be used as an enrichment broth, followed by subculture to a selective solid medium (e.g. Violet Red Bile with Glucose Agar).