FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences
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Product Details & Documents
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).
- An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate.
Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an 'inhibitor stop' will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
Specifications
Specifications
- Désignation:Fluor-de-Lys® Developer I concentrate
- Taille:300 µl
- Sufficient For:100 assays (50 µl reaction volume)
- Cat. No.:89160-284
- Concentration:20X concentrate after dilution (1X)
Specifications
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Catalog #: CA21905-026
Supplier: Kimberly-Clark