To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
- Description:pJY2 Plasmid
- Size:5 µg
- Cat. no.:89157-406
- Supplier No.:DW8720-0005
pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
- Liquid
- Prepared in 10 mM TRIS, pH 8.0, containing 1mM EDTA
- Store at –20 °C
The AGA arginine codon is rarely used in E. coli but is common in eukaryotic genes. The AGG arginine codon is also infrequently utilized in E. coli. As tRNAUCUArg decodes AGG when over-expressed, pJY2 is also likely to ameliorate problems associated with the expression of genes containing AGG codons.