To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Description:PTP1B Drug Discovery Kit
- Size:1 Kit
- Cat. no.:89156-558
- Storage Temperature:-80 °C
- No. of Assays/Kit:96 assays
- Supplier No.:AK822-0001
Specifications
About this item
The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
The kit includes human, recombinant PTP1B (residues 1-322; MW=37.4 kDa), expressed in E
PTP1B (protein tyrosine phosphatase-1B) is a ubiquitous, nontransmembrane protein tyrosine phosphatase, originally identified in human placenta.It is implicated in the negative regulation of insulin receptor signaling, and is a potential therapeutic target for treatment of type 2 diabetes and obesity. The phosphopeptide substrate supplied with this kit ("IR5", Prod. No. BML-P315, pTyr1158 (a.k.a. pTyr1146, mature peptide numbering)) contains sequence from the insulin receptor beta subunit domain that must be autophosphorylated to achieve full receptor kinase activation. This "activation loop" is the target of several protein phosphatase regulators of insulin signaling, including, notably, PTP1B. The PTP1B inhibitor suramin is supplied as a control for inhibitor detection. Suramin is a reversible and competitive inhibitor of PTP1B, with a Ki of 5.5 µM.
Store all components except the microtiter plate at -70°C for the highest stability. The PTP1B enzyme component must be handled particularly carefully in order to retain maximal enzymatic activity. Thaw it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be 'snap' frozen, e.g. in liquid nitrogen or a dryice/ethanol bath, and stored at -70°C. If only a few assays are to be performed each day, the PTP1B may be divided into several aliquots (best if ≥%10 µl)) to help minimize freeze/thaw cycles.
coli. The detection of free phosphate released is based on the classic Malachite green assay and offers the advantages of convenient, 1-step detection and excellent sensitivity, without radioactivity.