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Specifications
- Size:96 Wells
- Storage conditions:–80 °C (Long Term)
- Application:Fluorescent Detection, HTS Activity Assay, Cell-based Assays
- Cat. no.:89156-508
- Supplier No.:AK511-0001
Specifications
About this item
The HDAC1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC1 included in the kit.
- Useful for inhibitor screening or characterizing enzyme kinetics
- Includes optimal substrate selected from a panel of acetylated sites in p53 and histones
The Fluor de Lys® HDAC1 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC1. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.