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DPPIV Drug Discovery Kit, Enzo Life Sciences
Catalog # 89156-500
Supplier:  Enzo Life Sciences
CAS Number:  
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DPPIV Drug Discovery Kit, Enzo Life Sciences
Catalog # 89156-500
Supplier:  Enzo Life Sciences
Supplier Number:  BML-AK499-0001
CAS Number:  
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Specifications

  • Size:
    1 Kit
  • Cat. no.:
    89156-500
  • Storage Temperature:
    -80 °C
  • Application:
    Colorimetric detection, Fluorescent detection, HTS
    Activity assay
  • Supplier No.:
    AK499-0001

Specifications

About this item

The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.

DPPIV (DPP4, CD26) is a member of the class of proteases known as prolyl peptidases, which cleave proteins after proline residues and is thought to play roles in diabetes, cancer, and autoimmune diseases, making it a target for drug discovery.

The kit contains both a chromogenic substrate (H-Gly-Pro-pNA; Km=114 µM) and a fluorogenic substrate (H-Gly-Pro-AMC; Km=50 µM).

Cleavage of the p-nitroaniline (pNA) from the colorimetric substrate increases absorbance at 405 nm. The fluorimetric assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) moiety from the C-terminus of the peptide substrate, which increases its fluorescence intensity at 460 nm.

A DPPIV inhibitor, P32/98 (KI=130 nM12), is included for use as a control. Other DPP enzymes are available for specificity profiling.

Ordering information: Contents: DPPIV enzyme (human, recombinant), pNA substrate, Calibration standard (p-nitroaniline), AMC substrate, Calibration standard (7-Amino-4-methylcoumarin), Inhibitor, Assay Buffer, ½-volume clear microplate
Delivery: Kit includes DPPIV enzyme (human, recombinant), pNA substrate, Calibration standard (p-nitroaniline), AMC substrate, Calibration standard (7-Amino-4-methylcoumarin), Inhibitor, Assay Buffer, ½-volume clear microplate