Phosphothreonine detection set, Enzo Life Sciences
- L'article nécessite un contrôle de la température pour le stockage et la livraison avec des frais supplémentaires. Il n'est pas éligible au retour pour des raisons de sécurité et de qualité. Tenez compte des exigences avant d'acheter.
- Return Policy
Product Details & Documents
This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites.
Immunoprecipitation; Western Blot (20µl/lane (mini gel) for HRPO/DAB detection, or 5µl/lane (mini gel) for HRPO/ECL detection). Do not use milk/casein for blocking and dilution! Replace by BSA! We recommend to use 1% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.2% Tween 20 in 2x PBS.Optimal conditions must be determined individually for each application.
Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
: Positive control included. Contents: Contains 25µg each of clones:1E11 (Prod. No. ALX-804-168)4D11 (Prod. No. ALX-804-169)14B3 (Prod. No. ALX-804-170)
Spécifications
Spécifications
- Désignation:Phosphothreonine Detection Set
- Taille:1 Set
Spécifications
#: CA21905-026
Supplier: Kimberly-Clark