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Supplier: Promocell

Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of juvenile foreskin and adult skin (different locations)

Supplier: Promocell

Primary Human Pulmonary Artery Endothelial Cells (HPAEC) are isolated from human pulmonary arteries (the main pulmonary artery, left and right branches)

Supplier: Promocell

Primary Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are isolated from human pulmonary arteries and stain positive for smooth muscle α-actin

Supplier: Promocell

PromoCell hMSC-AT are harvested from normal human adipose tissue. The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts.

Supplier: Promocell

Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin. New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.

Supplier: Promocell

Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor. (Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.) The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.

Supplier: Promocell

Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process.

Supplier: Promocell

Primary Human Uterine Fibroblasts (HUF) are isolated from the uterine wall

Supplier: StemBioSys

CELLvo™ Healthy Human Chondrocytes (50-70y) are primary, uncultured cells isolated from recently-diseased cadaver cartilage from donors between 50 and 70 years old. Healthy status is determined by macroscopic inspection of the tissue using a modified outerbridge (1960) scale in order to distinguish healthy joints from those with signs of osteoarthritis. Cells are isolated by enzymatic digestion prior to cryopreservation.

Supplier: Promocell

Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.

Supplier: Promocell

Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of an adult heart

Supplier: Promocell

Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue

Supplier: Promocell

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request).

Supplier: StemBioSys

CELLvo ™ Human Cord Blood Endothelial Progenitor Cells have superior colony forming ability, greater vessel formation, and greater angiogenic potential compared to more common endothelial-type cells (HUVECs), CELLvo™ hCB-EPCs are the endothelial cell of choice for researchers.

Supplier: Promocell

Primary Human Renal Epithelial Cells (HREpC) are isolated from the adult kidney and stain positive for cytokeratin.

Supplier: Promocell

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs.

Supplier: Promocell

Primary Human Tracheal Epithelial Cells (HTEpC) are isolated from the surface epithelium of human trachea and stain positive for cytokeratin.

Supplier: Promocell

Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas.

Supplier: Promocell

PromoCell Human CD14+ Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated by positive selection with > 95% purity.

Supplier: Promocell

Primary Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from the arteries of the umbilical cord and stain positive for smooth muscle α-actin

Supplier: Promocell

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs. They are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation.

Supplier: Promocell

PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

Supplier: Promocell

Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.

Supplier: Promocell

Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue

Supplier: Promocell

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells. Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.

Supplier: Promocell

Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request). Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes.

Supplier: Biotium

Fluorescent dyes for tracking cell divisions by flow cytometry or tracing live cells by microscopy.

Supplier: LONZA PHARMA - BIOSCIENCE CA

Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response.

Supplier: MULTISPAN INC

Stable Cells

Supplier: EXPRESSION SYSTEMS LLC

Expression Systems offers insect cell lines adapted to serum-free suspension culture in either ESF 921 or ESF AF.