Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Fluorescent Wash Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.
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Radiance Plus Chemiluminescent Substrate, Azure Biosystems
Supplier: Azure Biosystems
Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.
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Transfer Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.
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Radiance Q Chemiluminescent Substrate, Azure Biosystems
Supplier: Azure Biosystems
Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability
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Protein Free Blot Blocking Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.
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Fluorescent Labeling Reagents, CypHer5E NHS Ester, Cytiva
Supplier: Cytiva
CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.
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Amersham ECL Western Blotting Start Detection Reagents, Cytiva
Supplier: Cytiva
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
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ECL Western Blotting ECF Substrate, Cytiva
Supplier: Cytiva
Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
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Pierce 1-Step™ Chloronaphthol Substrate Solution, Thermo Scientific
Supplier: Thermo Scientific
The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
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AEC Substrate Kit, Abcam
Supplier: Abcam
AEC substrate kit for use in IHC using HRP / peroxidase detection.
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Pierce™ Western Blotting Kits, Far-Western Blot Kit for Biotinylated Proteins, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Far-Western Blot Kit for Biotinylated Proteins provide a nonisotopic approach to far-Western analysis that can be performed either directly in the gel or on the membrane
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Pierce™ 1-Step™ Ultra TMB-Blotting Solution, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.
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Optiblot SDS-PAGE Sample Preparation Kit, Abcam
Supplier: Abcam
The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
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Pierce™ HRP Substrates for ELISA, OPD (o-phenylenediamine dihydrochloride), Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
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Alkaline Phosphatase chromogen (BCIP/TNBT), Abcam
Supplier: Abcam
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies.
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Antibody Diluent, Abcam
Supplier: Abcam
Antibody diluent (pH 7 - 7.8) is used to dilute primary antibodies to their working concentrations for use in immunohistochemistry (IHC). - Cited in over 15 publications - Reduces background and non-specific binding - Extends antibody stability up to 24 months at 2-8°C - Tris-buffered with stabilizing protein, wetting agent, antimicrobial agent, and green dye
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Catalog #: CA21905-026
Supplier: Kimberly-Clark