Vous avez recherché : Electrophoresis Reagents

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: Azure Biosystems

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and Western blotting transfer modules. The power supply can create and save protocols for all your gel and blotting experiments, and includes a pause/resume function for flexibility.

Supplier: G-Biosciences

Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis

Supplier: G-Biosciences

Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis

Supplier: New England Biolabs (NEB)

A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis

Supplier: New England Biolabs (NEB)

The Supercoiled DNA ladder contains 9 proprietary supercoiled plasmids, ranging in size from 2 to 10 kb, that are suitable for use as supercoiled molecular weight standards for agarose electrophoresis

Supplier: Lonza

During electrophoresis, these markers help monitor the efficiency of protein separation and allow accurate sizing of proteins in a sample. During Western blotting, they help confirm transfer has occurred from the gel to the membrane.

Supplier: VWR

Bags safely remove ethidium bromide from solution during overnight treatment

Supplier: VWR International

DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: VWR International

DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: Azure Biosystems

Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.

Supplier: VWR

13 fragments ranging from 100 to 3000 bp for easy band identification.

Supplier: VWR

RiboReady™ Color Micro RNA Ladder is made for precise sizing of RNA molecules in denaturing acrylamide gels

Supplier: Avantor

These DNA markers are used to determine size of unknown DNA fragments. If using within 2–3 days, they do not need to be refrigerated and can be stored right on a shelf. If storing for longer time periods, freezing is recommended.

Ship date is required.

Supplier: VWR

10 fragments ranging from 50 to 500 bp for easy band identification

Supplier: Azure Biosystems

Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.

Supplier: Azure Biosystems

Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.

Supplier: Azure Biosystems

Azure Blot Washing Buffer can be used with any Western blotting system.

Supplier: VWR

8 fragments ranging from 50 to 2000 bp for easy band identification

Supplier: Azure Biosystems

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

Supplier: Azure Biosystems

Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

Supplier: MilliporeSigma

Genomic DNAs are highly purified preparations of high molecular weight genomic DNA from a variety of organisms.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Enzo Life Sciences

Species Independent

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Supplier: Cytiva

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.