Vous avez recherché : Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.
Expand 7 Items
FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.
Expand 1 Items
SMART Digest™ Protein Digests Kits
Supplier: Thermo Scientific
Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.
Expand 29 Items
FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).
Expand 1 Items
Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
Expand 1 Items
Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific
Supplier: Thermo Scientific
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.
Expand 19 Items
BugBuster® HT Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E
Expand 3 Items
BugBuster® Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.
Expand 2 Items
VersaLyse™ Lysing Solution, 100 Tests, RUO
Supplier: Beckman Coulter
VersaLyse Lysing solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse/wash, wash/lyse and lyse/no-wash procedures with undiluted or diluted samples. The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K3EDTA anticoagulated blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).
Expand 1 Items
PopCulture® Reagent, MilliporeSigma
Supplier: MilliporeSigma
PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.
Expand 3 Items
BugBuster® 10X Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.
Expand 2 Items
BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.
Expand 2 Items
Lysonase™ Bioprocessing Reagent, EMD Millipore
Supplier: MilliporeSigma
Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.
Expand 2 Items
Lysing Matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used for breaking tough plant and animal samples.
Expand 10 Items
NP-40 Alternative, MilliporeSigma
Supplier: MilliporeSigma
NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.
Expand 2 Items
B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific
Supplier: Thermo Scientific
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
Expand 2 Items
Q-sep® QuEChERS Extraction Salts, Restek
Supplier: Restek
QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
Expand 7 Items
Pepsin Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Expand 2 Items
Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling
Expand 1 Items
Pierce™ Trypsin Protease, MS Grade, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
Expand 4 Items
Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific
Supplier: Thermo Scientific
Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
Expand 2 Items
Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.
Expand 1 Items
Protein Reducing Agents, G-Biosciences
Supplier: G-Biosciences
A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)
Expand 5 Items
Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose
Expand 1 Items
Pierce™ Detergent Removal Spin Column, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.
Expand 5 Items
Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific
Supplier: Thermo Scientific
Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.
Expand 1 Items
Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific
Supplier: Thermo Scientific
Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
Expand 1 Items
Factor Xa Protease, New England Biolabs
Supplier: New England Biolabs (NEB)
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg
Expand 2 Items
ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Expand 2 Items
Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific
Supplier: Thermo Scientific
The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.