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Protein Purification Reagents

Protein Purification Reagents

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

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Pierce™ Immobilised TCEP Disulfide Reducing Gel, Thermo Scientific

Pierce™ Immobilised TCEP Disulfide Reducing Gel, Thermo Scientific

Supplier: Thermo Scientific

Immobilised TCEP Disulfide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.

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Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), Thermo Scientific

Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), Thermo Scientific

Supplier: Thermo Scientific

Pierce™ HABA (4'-hydroxyazobenzene-2-carboxylic acid), a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

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Pierce™ Pegylation Reagents, Methyl-PEG-NHS-Maleimide, Thermo Scientific

Pierce™ Pegylation Reagents, Methyl-PEG-NHS-Maleimide, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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Pierce™ Protein Stabilizing Cocktail, Thermo Scientific

Pierce™ Protein Stabilizing Cocktail, Thermo Scientific

Supplier: Thermo Scientific

A versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.

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HRP Stability Conjugate Reagent

HRP Stability Conjugate Reagent

Supplier: Abcam

Abcam's HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution.

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Diluent M Concentrate (10x)

Diluent M Concentrate (10x)

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Pierce™ Pegylation Reagents, MS(PEG)n Methyl-PEG-NHS-Esters, Thermo Scientific

Pierce™ Pegylation Reagents, MS(PEG)n Methyl-PEG-NHS-Esters, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3 x 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Pierce™ High Sensitivity Streptavidin-HRP, Thermo Scientific

Pierce™ High Sensitivity Streptavidin-HRP, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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SulfoLink™ Coupling Resin, Thermo Scientific

SulfoLink™ Coupling Resin, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

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Pierce™ Immobilized p-Aminophenyl Phosphoryl Choline Agarose, Thermo Scientific

Pierce™ Immobilized p-Aminophenyl Phosphoryl Choline Agarose, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids.

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Protein Deglycosylation Mix II, New England Biolabs

Protein Deglycosylation Mix II, New England Biolabs

Supplier: New England Biolabs (NEB)

The Protein Deglycosylation Mix II contains all of the enzymes, reagents, and controls needed to remove all N-linked and simple O-linked glycans as well as some complex O-linked glycans.

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EnGen® Spy Cas9 NLS, New England Biolabs

EnGen® Spy Cas9 NLS, New England Biolabs

Supplier: New England Biolabs (NEB)

EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.

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PureCube NTA Agarose

PureCube NTA Agarose

Supplier: Cube Biotech

PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

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PureCube Glutathione Agarose

PureCube Glutathione Agarose

Supplier: Cube Biotech

The protein purification with the PureCube Glutathione Agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

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Pierce™ Streptavidin, Maleimide-Activated, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Maleimide-Activated Streptavidin conjugate include streptavidin in a purified form, activated for crosslinking to sulfhydryl groups containing molecules.

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HighSpec Compact Cartridge Rho1D4

HighSpec Compact Cartridge Rho1D4

Supplier: Cube Biotech

The HighSpec Rho1D4 Compact cartridge is a pre-packed FPLC column containing our HighSpec Rho1D4 Agarose resin.

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