You searched for: Cloning Kits and Ligation

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Supplier: New England Biolabs (NEB)

SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

Supplier: New England Biolabs (NEB)

The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)

Supplier: New England Biolabs (NEB)

RtcB Ligase is expressed as a His-tagged fusion in E. coli.

Supplier: ReVacc Scientific

This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Supplier: New England Biolabs (NEB)

The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

Supplier: New England Biolabs (NEB)

Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

Supplier: New England Biolabs (NEB)

Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Supplier: New England Biolabs (NEB)

T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Supplier: New England Biolabs (NEB)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

Supplier: New England Biolabs (NEB)

The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.

Supplier: New England Biolabs (NEB)

NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

Supplier: New England Biolabs (NEB)

Gibson Assembly was developed by Dr

Supplier: New England Biolabs (NEB)

A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.