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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

10X TBE (Tris-Borate EDTA) pH 8.3

Supplier: GROWCELLS

TBE is the most commonly used buffer for DNA and RNA electrophoresis

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RNA Gel Buffer 10X (MOPS buffer), Quality Biological

RNA Gel Buffer 10X (MOPS buffer), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

MOPS buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane.

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10X TAE (Tris-Acetate EDTA), pH 8.3

Supplier: GROWCELLS

TAE buffer is used in agarose gel electrophoresis and Polyacrylamide gels both as a buffer and a component of a gel

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50X TAE (Tris-Acetate EDTA), pH 8.3

Supplier: GROWCELLS

TAE buffer is used in agarose gel electrophoresis and Polyacrylamide gels both as a buffer and a component of a gel

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IPG Buffers, Cytiva

IPG Buffers, Cytiva

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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Electrophoresis Running Buffers, G-Biosciences

Supplier: G-Biosciences

G-Biosciences offers many electrophoresis running buffers including: TAE Running Buffer [50X], TBE Running Buffer [10X], TBE-Urea Sample Buffer, MOPS Running Buffer [10X], and MOPS/ SDS Running Buffer [20X]

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Pierce™ Electrophoresis Running Buffers, Thermo Scientific

Pierce™ Electrophoresis Running Buffers, Thermo Scientific

Supplier: Thermo Scientific

These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.

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Protein Running Buffer 10X, Quality Biological

Protein Running Buffer 10X, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Protein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis.

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Chemi Blot Blocking Buffer, Azure

Chemi Blot Blocking Buffer, Azure

Supplier: Azure Biosystems

Azure Chemi Blot Blocking Buffer, Ready-to-use block that enhances chemiluminescent signal, for use with chemiluminescent Western blots to enhance signal and lower background. Size: 500mL

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Corning® 10X TAE Buffer, Liquid, Corning

Corning® 10X TAE Buffer, Liquid, Corning

Supplier: Corning

Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer

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10X Tris-Glycine

Supplier: Rockland Immunochemical

Tris-Glycine Running Gel buffer for polyacrylamide gel electrophoresis - without SDS (sodium dodecyl sulfate)

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DNA Gel Loading Solution, 5X, Quality Biological

DNA Gel Loading Solution, 5X, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

DNA Gel Loading Solutio, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer.

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TBE 10X, Quality Biological

TBE 10X, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water.

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ProSieve™ EX Buffer

ProSieve™ EX Buffer

Supplier: Lonza

ProSieve™ EX Buffers accelerate speed and performance on separation or transfer of most Tris-Glycine Gels without any compromise to resolution or methods.

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SSPE Buffer, 20X, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.

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20X SSC Buffer (Standard Sodium Citrate)

Supplier: GROWCELLS

Concentrated sodium chloride-sodium citrate buffer functions by providing high ionic strength to optimize the rate of probe annealing to target nucleic acid

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20X SSC pH 7.0

Supplier: Rockland Immunochemical

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays).

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Corning® TBE Buffer, Liquid, 10X, Corning

Corning® TBE Buffer, Liquid, 10X, Corning

Supplier: Corning

TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis

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10X Tris-Borate-EDTA (TBE) Buffer

Supplier: Rockland Immunochemical

TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology.

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RNA Gel Loading Solution 10X, Quality Biological

RNA Gel Loading Solution 10X, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989)

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10X Tris-Acetate-EDTA (TAE)

Supplier: Rockland Immunochemical

10X Tris-Acetate-EDTA (TAE) Buffer

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10X PBS pH 7.2

Supplier: Rockland Immunochemical

10X Phosphate buffered saline (PBS) pH 7.2

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Tris-Acetate-EDTA (TAE) 50X, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA.

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Tris Glycine Transfer Buffer 10X (Transblotting Buffer 10X), Quality Biological

Tris Glycine Transfer Buffer 10X (Transblotting Buffer 10X), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

TRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X).

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Customized SDS Protein Gel Loading Solution, Quality Biological

Customized SDS Protein Gel Loading Solution, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

This is a special formulation of loading solution for protein gel electrophoresis.

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TAE Buffers, Quality Biological

TAE Buffers, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.

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1X TBS Buffer with 1% Casein

Supplier: Rockland Immunochemical

Tris buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.

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SDS Protein Gel Loading Solution, 2X, Quality Biological

SDS Protein Gel Loading Solution, 2X, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

"SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front.

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SSC, 20X Buffer, Quality Biological

SSC, 20X Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization

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