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Cell Culture Buffers

Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

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VWR® Sodium Phosphate 0.1 M, pH 7.0 Buffer

VWR® Sodium Phosphate 0.1 M, pH 7.0 Buffer

Supplier: VWR International

Made with sodium phosphate monobasic monohydrate, sodium hydroxide solution and deionized water.

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VWR® Blood Bank Saline Solutions

VWR® Blood Bank Saline Solutions

Supplier: VWR International

Blood bank saline is pH-controlled for a pH between 6.5 and 7.0.

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VWR® 3D Clear Washing Buffer 10X

Supplier: VWR International

VWR® Washing Buffer, 10X, 3D Clear

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VWR® TRIS Buffer, 0.1 M Solution, pH 7.4

VWR® TRIS Buffer, 0.1 M Solution, pH 7.4

Supplier: VWR

Cell culture buffer, TRIS Buffer Solution 0.1M, pH 7.4

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VWR® Phosphate Buffered Saline, Ultra Pure Grade

VWR® Phosphate Buffered Saline, Ultra Pure Grade

Supplier: VWR

1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.8 mM Phosphate buffer

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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade

VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade

Supplier: VWR

1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.

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VWR® TRIS Buffer, 1 M Solution, pH 8.0

VWR® TRIS Buffer, 1 M Solution, pH 8.0

Supplier: VWR

Tris (hydroxymethyl) aminomethane

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Phosphate Buffer

Phosphate Buffer

Supplier: Ward's Science

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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

Supplier: VWR

Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.

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VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade

VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade

Supplier: VWR

1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer.

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Phosphate Buffered Saline

Phosphate Buffered Saline

Supplier: Ward's Science

Phosphate Buffered Saline

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Buffer Solution

Buffer Solution

Supplier: Ward's Science

Buffer solution has a shelf life of 24 months

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VWR® Ultra Pure Grade Phosphate Buffered Saline

VWR® Ultra Pure Grade Phosphate Buffered Saline

Supplier: VWR

1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer

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Biofluid & Cell Buffer (Red), Zymo Research

Biofluid & Cell Buffer (Red), Zymo Research

Supplier: Zymo Research

Cell culture buffer

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Dulbecco's phosphate-buffered saline (DPBS), Mediatech

Dulbecco's phosphate-buffered saline (DPBS), Mediatech

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6

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PBS 10X-Dulbecco's Phosphate Buffered Saline Solution

PBS 10X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

PBS 10X Dulbecco's Phosphate buffered saline solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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Corning® HEPES buffer solution (1 mol/L)

Corning® HEPES buffer solution (1 mol/L)

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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HEPES Buffer Solution 1 M

HEPES Buffer Solution 1 M

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO₂ incubator

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Phosphate Buffered Saline (PBS), Quality Biological

Phosphate Buffered Saline (PBS), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

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Corning® PBS (Phosphate-Buffered Saline), Corning

Corning® PBS (Phosphate-Buffered Saline), Corning

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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Corning® Tris, 10X Buffered Saline, Liquid, Corning

Corning® Tris, 10X Buffered Saline, Liquid, Corning

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range

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Corning® HBSS (Hanks Balanced Salt Solution), Corning

Corning® HBSS (Hanks Balanced Salt Solution), Corning

Supplier: Corning

Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.

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Corning® 3M™ Sodium Acetate, Corning

Corning® 3M™ Sodium Acetate, Corning

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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PBS 1X-Dulbecco's Phosphate Buffered Saline Solution

PBS 1X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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Dulbecco's Phosphate Buffered Saline (DPBS), Quality Biological

Dulbecco's Phosphate Buffered Saline (DPBS), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

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Corning® Sodium Bicarbonate, Corning

Corning® Sodium Bicarbonate, Corning

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment

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Corning® Phosphate-Buffered Saline (PBS), 10X Concentrate, pH 7,4, Molecular Biology Grade

Corning® Phosphate-Buffered Saline (PBS), 10X Concentrate, pH 7,4, Molecular Biology Grade

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

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HEPES Buffer (1 M), pH 7.3, Quality Biological

HEPES Buffer (1 M), pH 7.3, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research.

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Phosphate Buffer

Phosphate Buffer

Supplier: HARDY DIAGNOSTICS CA

Phosphate Buffer, 500ml fill, 500ml polycarbonate bottle, for the preparation of specimens for Acid-Fast Bacilli culture

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Neutralizing Buffer, Hardy Diagnostics

Neutralizing Buffer, Hardy Diagnostics

Supplier: Hardy Diagnostics

Neutralizing Buffer allows the bacteriostatic effects of chlorine and quaternary ammonia compounds

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